The test works on the principle of lateral flow immuno-chromatography to detect Influenza A and B antigens. When the sample and buffer are applied to the sample pad, the liquid migrates through the conjugate pad, which contains colloidal gold conjugated to monoclonal antibodies for both Influenza A and Influenza B. The antigens from the sample bind to these antibodies and form visible lines in the respective zones (A and B).
If the sample contains Influenza A or Influenza B antigens, a visible color line will form in the corresponding test zone (A or B). If no antigens are present, no color lines will appear. A control line (C) will always appear to indicate proper test functioning.