The test uses an immunochromatographic principle to detect HIV-1/HIV-2 antibodies in a sample. When the sample containing the antibodies and the buffer are added to the sample pad, the liquid mixture migrates to the conjugate pad, which contains latex nanoparticles embedded with HIV antigens. The HIV-1/HIV-2 antibodies from the sample bind to the conjugate and then to the immobilized HIV antigens in the test zone, forming a visible colored line.